Journal: bioRxiv
Article Title: In vivo imaging of T-cell coregulator B7-H4 reveals protumor macrophage status in prostate cancer
doi: 10.1101/2024.09.28.615608
Figure Lengend Snippet: B7-H4 expression in prostate cancer cell lines. A) Western blot protein bands showing the expression level of B7-H4 protein in human DU145 and murine TRAMP-C2 cell lines. The bar graph shows the relative expression of B7-H4 protein levels between DU145 cell and TRAMP-C2 cell calculated as fold differences using ImageJ software. Data are displayed as mean data of n = 3 per group and standard deviation. B) Immunofluorescence staining of B7-H4 protein in prostate cancer cell lines with DAPI (blue) nuclear staining and B7-H4 (green, Alexa-488). Scale bar = 100µm.
Article Snippet: Human and mouse prostate cancer cell lines DU145 and TRAMP-C2 were purchased from the American Type Culture Collection (ATCC).
Techniques: Expressing, Western Blot, Software, Standard Deviation, Immunofluorescence, Staining